There’s a reason why the job outlook for chemists is supposed to grow by 6% over the next decade. We rely on these professionals to research chemical components that can aid everything from medicine to manufacturing.
An essential part of this research involves column chromatography. But precisely what is column chromatography? And what are the applications?
If you want to learn the answer to this question, and more, you’re in the right place. In this column chromatography guide, we’ll teach you everything you need to know about this important subject.
What Is Chromatography?
Before we learn about column chromatography, it’s important to first answer the questions, What is chromatography? Chromatography is the process in which the components of a mixture are separated, identified, and purified.
In other words, it’s a biophysical technique that consists of a mobile phase and an absorbent phase. The separation occurs when the mobile phase comes in contact with the unmoving absorbent phase.
What Is Column Chromatography?
Column chromatography is a subcategory technique that is used when you want to isolate a substance. Basically, the substance is applied to the top part of a column that’s loaded with an absorbent phase.
From there, the substances begin passing through the column at different rates. These rates ultimately depend on the affinity that each substance has for both the absorbent phase and the solvent mixture in which they’re collected.
If you want to learn more about the equipment that goes into column chromatography, you can check out this website here (chromtech.com).
Benefits of Column Chromatography
The main benefit of column chromatography is that any type of mixture can be separated with it. In addition to this, any quantity can be separated. With column chromatography, you also get a much wider choice when it comes to your mobile phase.
Also, when it’s used in a preparative type, the sample you use can be reused once you separate it. Lastly, automation is possible with column chromatography. So you won’t need to have as many assistants on hand.
What Are the Applications?
The main use of column chromatography is the separation of compounds from mixtures. However, it can also be used to remove impurities from substances.
It’s helpful for isolating both active constituents and any metabolites found in biological fluids. Lastly, it can be used to find the rough estimates of drugs in a mixture.
Appreciate Learning About Chromatography? Keep Reading
We hope this guide helped you answer the question, What is column chromatography? As you can see, chromatography is a pretty niche subject. But it’s still a vital part of separating mixtures.
So, if you’re a chemist or a biologist, it’s important to know about it. Want similar science subjects? If the answer is yes, you’re in the right place. Keep exploring our site to find tons of similar articles to choose from.
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